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Journal of Nanostructure in Chemistry (100 μg mL−1), the MMP-1 expression was decreased to 59% of the normal value. On the contrary, the treatment with S-AgNPs (1, 10 and 100 μg mL−1), promoted the expression of procollagen type I in a dose-dependent manner. Interest- ingly, our results suggest that S-AgNPs synthesized from comfrey suppressed UVB-induced overexpression MMP-1, IL-6 while enhancing the expression of procollagen type 1. Conclusion The present study demonstrated the biological synthesis of irregular-shaped S-AgNPs using S. officinale leaf extract. The method was fast, simple, eco-friendly and cheap. Fur- ther, the S-AgNPs prevented UVB-induced photoaging in HaCaT cells. S-AgNPs alleviated the UVB-induced skin damage by suppressing the production of MMP1 and IL-6. On the other hand, S-AgNPs increases the production of procollagen type 1. These findings suggest that S-AgNPs are a promising agent in protection against photoaging, however, further studies including in vivo needs to be done. Acknowledgements This work was conducted under the industrial infrastructure program (Grant no. N0000888) for fundamental technol- ogies which is funded by the Ministry of Trade, Industry, and Energy (MOTIE, Korea). Open Access This article is distributed under the terms of the Crea- tive Commons Attribution 4.0 International License (http://creativeco mmons.org/licenses/by/4.0/), which permits unrestricted use, distribu- tion, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. References 1. Saha, J., Begum, A., Mukherjee, A., Kumar, S.: A novel green synthesis of silver nanoparticles and their catalytic action in reduction of methylene blue dye. Sustain. Env. Res. 27, 245–250 (2017) 2. 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Du, J., Singh, H., Yi, T.H.: Antibacterial, anti-biofilm and anti- cancer potentials of green synthesized silver nanoparticles using benzoin gum (Styrax benzoin) extract. Bioprocess Biosyst Eng. 39, 1923–1931 (2016) 13PDF Image | green silver nanoparticles from leaf used as UVb protection
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