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Fig. 8 Effect of S-AgNPs on UVB-induced MMP-1 (a), and IL-6 (b) secretion in HaCaT cells. HaCaT cells were either irradiated with 100 mJ cm−2 or nonirradiated, and then all cells were treated with the indicated concentration of S-AgNPs (1, 10 and 100 μg mL−1) for 24 h. Data are presented as the calculated percentage of control group reduction of type I collagen is considered the primary cause of skin aging [27]. In this study, we further investigated the effect of S-AgNPs on MMP-1 and procollagen type 1 mRNA expression in HaCaT cells by RT-PCR analysis. UVB irra- diation significantly altered the mRNA levels of MMP-1 and expression and are presented as the mean±SD. ###p<0.001, com- pared with the nonirradiated group (normal); *p<0.05, **p<0.01, ***p<0.001, compared with the only ultraviolet B (UVB)-irradiated group (control) procollagen type 1. As shown in Fig. 9, there was a low basal mRNA expression of MMP-1 in non-UVB-irradiated cells. The MMP-1 mRNA level was significantly elevated in UVB- irradiated cells, compared with nonirradiated cells. Con- sistent with the ELISA result, in the presence of S-AgNPs Fig. 9 Effect of AgNPs on MMP-1 (a), and procollagen type 1 (b) mRNA expression. Equimolar quantities of mRNA were quanti- fied relative to GAPDH. Data are presented as the mean±SD. ###p<0.001, compared with the nonirradiated group (normal); *p<0.05, **p<0.01, compared with the only ultraviolet B (UVB)- irradiated group (control) Journal of Nanostructure in Chemistry 13PDF Image | green silver nanoparticles from leaf used as UVb protection
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