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Plants 2022, 11, 2379 2.8. Principal Component Analysis (PCA) The results of Pearson’s correlation analysis were further confirmed by PCA-Biplot that showed that synthesis of antioxidants in barley has a direct relationship with plant growth, which on the other hand, is compromised by the excessive production of ROS non-enzymatic antioxidants with morphological attributes (SL, RL, SFW, SDW, RFW, RDW), H2O2 and MDA contents, we performed PCA analysis. The two components of and lipid peroxidation (Figure 6A,B). To infer the relationship of enzymatic and A positive correlation was found among enzymatic antioxidants with some exceptions on tchuembuelhaatilvfeofvlaeraifaAncPeXin(FDigiumre15aBn)d.Dim2fornon-enzymaticandenzymaticantioxidants accounts for 83% and 80%, respectively (Figure 6A,B). The both PCAs exhibited that the 2.8. Principal Component Analysis (PCA) majority of non-enzymatic and all enzymatic antioxidants, ROS (H2O2) and lipid peroxTihdeatrieosnul(tMsoDfAP)eaarrseonf’osucnodrreinlatDioinma1n.aSlyositshweefriresftucrothmeproconnenfitrmeadybbyePCnaAm-Beidplaost tahnatitosxhiodwanetdstchaavtesnygnetrheasnisdosfaalnintitoyxitdoalnertasninceb,awrlheyilehainsaDdiimre2c,tarellathioensghroipwwthithreplalatendt gmrorwptho,lwoghicahloanttrthibeuotethsearrheapnrdes,eisncteodm(pFriogmuriese6dAb,By)t.he excessive production of ROS and lipidTpheeroaxnidgaletiboentw(FeigeunrLeH6A2O,B2)a.nTdo LinPfreorlt;hLeMreDlaAtioansdhLipGoSfSeGn;zRyHm2aOti2caandRnMonD-eAn;zLyMmaDtAic andtioLxGidSaSnGts(wFigthurmeo6rAp)hoanlodgiScFaWl a,ttSriDbWut,esR(FSWL,,RLD,WSF,WS,LSaDnWd,RLFWan,dRDinWP)C, HA O(Figaunrde 22 M6AD,BA) schoonwteendts,thwatetpherfeoremxiestdaPsCtAronagnaplyositsi.veT.hIet htwasobceoemnpoobnsernvtsedofthPaCtAR,Hi.2eO.,2 PaCnd1 aRnGdSSPGC2ewxheirbeitredprneosenretleadtiaosnsDhiimp1baentwdeDenimt2h,ersesptwecotivcoelmy.pTohnenctusm. Suilmatiilvaerlyv,artihaencdeaitna 10 of 18 PCA, i.e., PC1 and PC2 were represented as Dim1 and Dim2, respectively. The Dim1 and Dim2 for non-enzymatic and enzymatic antioxidants accounts for 83% and 80%, presented in Figure 6B revealed that there is a strong positive correlation between LH2O2 respectively (Figure 6A,B). The both PCAs exhibited that the majority of non-enzymatic and and LAPX; LTSP, LSOD, LPOD, LGR, RTSP, RAPX, RCAT, RAPX and RSOD, RH2Os and all enzymatic antioxidants, ROS (H O ) and lipid peroxidation (MDA) are found in Dim1. RPOD and LH2O2 and LCAT, w2hil2e the angle between LH2O2 and morphological So the first component may be named as antioxidant scavenger and salinity tolerance, attributes showed that a strong negative correlation exists. The representation of RMDA while in Dim2, all the growth related morphological attributes are presented (Figure 6A,B). showed that it had a little contribution in both PCAs (Figure 6A,B). Figure 6. Principal component analysis (PCA) showing the relationship of different morphological, Figure 6. Principal component analysis (PCA) showing the relationship of different morphological, total soluble protein, amino acids, H2O2 and MDA contents with non-enzymatic (A) and enzymatic 22 (B)anttiioxxiidaannttssooffbabralerlyeygegneontyoptyepsemsomduodlauteladtebdy abpypalipcaptliocnatoiofnsiloicfosnilitchoronutghhrorouogthinrgomoteindgiamunedeiar under salinity stress. SL: shoot length; RL: root length; SFW: shoot fresh weight; RFW: root fresh salinity stress. SL: shoot length; RL: root length; SFW: shoot fresh weight; RFW: root fresh weight; weight; SDW: shoot dry weight; RDW: root dry weight; LTSP: leaf total soluble protein; RTSP: root SDW: shoot dry weight; RDW: root dry weight; LTSP: leaf total soluble protein; RTSP: root total soluble total soluble protein; LAA: leaf free amino acids; RAA: root free amino acids; LH2O2: leaf hydrogen protein; LAA: leaf free amino acids; RAA: root free amino acids; LH2O2: leaf hydrogen peroxide; peroxide; RH2O2: root hydrogen peroxide; LMDA: leaf malondialdehyde; RMDA: root RH2O2: root hydrogen peroxide; LMDA: leaf malondialdehyde; RMDA: root malondialdehyde; malondialdehyde; LTG: leaf total glutathione; RTG: root total glutathione; LGSH: leaf reduced LTG: leaf total glutathione; RTG: root total glutathione; LGSH: leaf reduced glutathione; RGSH: root glutathione; RGSH: root reduced glutathione; LGSSG: leaf oxidized glutathione; RGSSG: root reduced glutathione; LGSSG: leaf oxidized glutathione; RGSSG: root oxidized glutathione; LTPhen: oxidized glutathione; LTPhen: leaf total phenolics; RTPhen: root total phenolics; LAsA: leaf leaf total phenolics; RTPhen: root total phenolics; LAsA: leaf ascorbic acid; RAsA: root ascorbic acid; ascorbic acid; RAsA: root ascorbic acid; Ltoco: leaf α-tocopherol; Rtoco: root α-tocopherol; LProl: Lletaofcop:rloealifnαe;-toRcporpohl:erorol;oRttopcro:lirnoeo;tαL-StDoc:oplehaefroslu;LpPeroxl:idleeafdpirsomliuntea;sRep;rRolS:OroDo:tprrooltinesu;LpSeDro:xliedaef sduispmeruotxaisdee; LdAisPmXu:tlaesaef;aRsScoOrDba:treopotersouxpiedraosxei;dReAdPisXm: ruotoastea;sLcoArPbXat:elepaefraosxciodrabsaet;eLpCeAroTx:ildeafsec;aRtaAlaPsXe;: RCAT: root catalase; LPOD: leaf peroxidase: RPOD: root peroxidase: LGR: leaf glutathione root ascorbate peroxidase; LCAT: leaf catalase; RCAT: root catalase; LPOD: leaf peroxidase: RPOD: reductase; RGR: root glutathione reductase. root peroxidase: LGR: leaf glutathione reductase; RGR: root glutathione reductase. The angle between LH2O2 and LProl; LMDA and LGSSG; RH2O2 and RMDA; LMDA and LGSSG (Figure 6A) and SFW, SDW, RFW, RDW, SL and RL and in PCA (Figure 6A,B) showed that there exist a strong positive. It has been observed that RH2O2 and RGSSG exhibited no relationship between these two components. Similarly, the data presented in Figure 6B revealed that there is a strong positive correlation between LH2O2 and LAPX; LTSP, LSOD, LPOD, LGR, RTSP, RAPX, RCAT, RAPX and RSOD, RH2Os and RPOD and LH2O2 and LCAT, while the angle between LH2O2 and morphological attributes showedPDF Image | Silicon-Induced Mitigation of NaCl Stress in Barley
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