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16 Graphene – Synthesis, Characterization, Properties and Applications Lastly, we have one comment that the graphene is a promising material supporting bio- molecules for TEM observations. In Fig. 15 and Fig. 16, we showed the transmission electron diffraction patterns of a single-layer graphene measured with different electron energies of 5, 1 and 0.5 kV. With decreasing the electron energy, the ratios of diffraction spot intensity to the background intensity, and the (00) spot intensity to the (01) intensity became small, because the sensitivity of light elements change. Fig. 15 are the SEM image of single graphene sheet at 5 kV and its Low Energy- Transmission Electron Diffraction (LETED) pattern at 5kV. Compared with TEM image, we can clearly observe the graphene surface by SEM. Fig. 15. The SEM image of single graphene sheet at 5 kV and its LETED pattern (upper right). Fig. 16 shows the LETED pattern of graphene at (a) 1 kV and (b) 500 V. When we decreased the electron energy down from 1 kV to 500 V, the intensity of the (10) spot increased compared with that of the (00) spot because of large elastic scattering cross section of electrons by graphene. Adsorbed molecules on graphene sheet also increase the intensity of the diffuse scattering. Fig. 17 is (a) the SEM image of folding double graphene sheet and its LETED pattern at (b) 4 kV and (c) 2kV. When we decreased the electron energy down from 4kV to 2kV, the additional satellite spots (white circles in image (b)) due to the double diffraction appeared in the patterns because of the large elastic scattering cross section.PDF Image | GRAPHENE SYNTHESIS CHARACTERIZATION PROPERTIES
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