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Polymers 2020, 12, 440 12 of 15 Table 1. Test results of films’ contact sterilization ability. E. coli Bacterial Growth 1 Sterilization Rate (%) 1 ”+” meant growth and ”-” meant not growth. 4. Conclusions In summary, this study investigated the preparation of Ag-NPs solution using in situ reduction and one-pot synthesis of cellulose-Ag-NPs composite films. Due to the aggregation effect of Ag-NPs and the coupling effect of MPTS, the process of Ag-NPs growth and cross-linking with cellulose during the one-pot synthesis were conducted simultaneously. A uniform Cellulose-Ag-NPs composite film was successfully prepared. The CANF have better tensile properties, thermal stability, and antibacterial ability because of Ag-NPs. The large number of Ag-NPs uniformly distributed on the surface of the CANF0.04 makes it easier for the Ag-NPs attached to the bacterial cell wall and kill the bacteria that gives the film good antibacterial properties. In addition, the amount of residual LiCl/DMAc system in the film was low, and the amount of silver diffusion was lower than the dose that produces cytotoxicity. Therefore, the silver-loaded cellulose film obtained in the experiment may have potential for applications in food packaging and bacterial barrier. Author Contributions: S.-L.X. conceived the original concept. Q.-Y.C. conducted experiments and the investigation as well as wrote the manuscript. S.Q.S. and L.-P.C. directed and revised the manuscript. All authors have read and agreed to the published version of the manuscript. Sample S. aureus Bacterial Growth 1 Sterilization Rate (%) + 0 CANF0.04 - 100 - 99.9 Blank control RCF + 0 + 0 + 0 Funding: This research was funded by The National Key R&D Program of China, grant number 2017YFD0601004. Polymers 2020, 12, 440 13 of 15 Conflicts of Interest: The authors declare no conflicts of interest. Appendix A TheGCcurvesofDMAcstandardsolution(100μμg/g),RCFandCANF0.04 obtainedbyGC‐-MS 0.04 scanning as shown in the Figure A1. DMAc 4.235 100.00 DMAc 100 μg/g 7.937 0.66 RC 4.496 4.17 10.940 100.00 7.937 62.91 9.284 16.68 CAN4 10.874 100.00 4.132 7.941 9.284 0.43 0.50 0.50 4 6 8 10 12 4 6 8 10 12 4 6 8 10 12 Time (min) Time (min) Time (min) (a) (b) (c) Figure A1. GC curves of DMAc, RCF, and CANF0.04: (a) GC curve of DMAc; (b) GC curve of RCF; (c) Figure A1. GC curves of DMAc, RCF, and CANF0.04: (a) GC curve of DMAc; (b) GC curve of RCF; GC curve of CANF0.04. (c) GC curve of CANF0.04. References 1. Marambio‐-Jones, C.; Hoek, E..M..V. A review of the antiibacterial effffects of siilver nanomaterials and potential iimplicationsforhumanhealthandtheenvironment.J..Nanopart..Reess..22001100,,1122,,11553311––1155511.. [CrossRef] 2. Ivask, A.; Kurvet, I.; Kasemets, K.; Blinova, I.; Aruoja, V.; Suppi, S.; Vija, H.; Käkinen, A.; Titma, T.; Heinlaan, M.; et al. Size‐Dependent Toxicity of Silver Nanoparticles to Bacteria, Yeast, Algae, Crustaceans and Mammalian Cells In Vitro. PLoS ONE 2014, 9, e102108. 3. Liu, W.; Wu, Y.; Wang, C.; Li, H.C.; Wang, T.; Liao, C.Y.; Cui, L.; Zhou, Q.F.; Yan, B.; Jiang, G.B. Impact of silver nanoparticles on human cells: Effect of particle size. Nanotoxicology 2010, 4, 319–330. 4. Gliga, A.R.; Skoglund, S.; Wallinder, I.O.; Fadeel, B.; Karlsson, H.L. Size‐dependent cytotoxicity of silver nanoparticles in human lung cells: The role of cellular uptake, agglomeration and Ag release. Part. FibrePDF Image | One-Pot Synthesis of Antibacterial Silver Nanoparticle
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