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Antibiotics 2018, 7, 76 3 of 12 Figure 1. Phytophthora cinnamomi growth inhibition assays with the nanocomposites based on the chitosan oligomers in hydroalcoholic solution preparation medium. From left to right: Control (no treatment) and treatments with AgNPs combined with gallic acid, silymarin, ferulic acid and curcumin inclusion compounds. From top to bottom: 125 μg·mL−1, 250 μg·mL−1, and 500 μg·mL−1 concentrations. Only one repetition per treatment is shown. Figure 2. P. cinnamomi growth inhibition assays with the nanocomposites based on the deep eutectic solvent preparation medium. From left to right: Control (no treatment) and treatments with AgNPs combined with gallic acid, silymarin, ferulic acid and curcumin inclusion compounds. From top to bottom: 125 μg·mL−1, 250 μg·mL−1 and 500 μg·mL−1 concentrations. Only one repetition per treatment is shown. The blue background in the control and curcumin-treated samples is due to the blue color of the paper on which the plates were on. As shown in Figure 3, the increase in the concentration of the inclusion complexes from 125 μg·mL−1 to 500 μg·mL−1 resulted in a reduction in the radial growth of the mycelium in all cases. It may be observed that 100% mycelial growth inhibition occurred with the COS medium at the highest concentration of 500 μg·mL−1 for the composites with gallic acid, ferulic acid and curcumin (but not for silymarin). On the other hand, at lower concentrations (125 and 250 μg·mL−1), silymarin and ferulic acid-based treatments were more effective than those based on gallic acid and curcumin. As regards the nanocomposites with a DES preparation medium, total inhibitory activities were attained for the composites based on the four polyphenols under study concentrations of 250, and 500 μg·mL−1. Further, at the lowest concentration of 125 μg·mL−1, the antifungal performance ofPDF Image | Silver Nano Polyphenol Inclusion Compounds Composites
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