PDF Publication Title:
Text from PDF Page: 003
Journal of Nanostructure in Chemistry perennial plant found in Asia, Europe, and North America. S. officinale has been commonly used in folk medicine for the treatment of diarrhea, bronchitis, tuberculosis, ulcers, and hemorrhoids. The plant extract was reported to be used as an ointment to promote the wound healing, reduce the inflam- mation, for the treatment of broken bones, tendon damages, painful joints, and muscles [17]. Although Comfrey also contains dehydropyrrolizidine alkaloids (DHPAs) because of which its internal application is not recommended [18]. Recently, the root extract of comfrey has shown to have anti- oxidant and proliferative activity [19]. However, there is no report of AgNPs synthesized from comfrey on UVB-induced photodamage. Here, we hypothesize that the AgNPs syn- thesized from Comfrey leaf extract could be used for pro- tection against UVB-induced skin photoaging. To test this hypothesis, markers of skin photoaging, MMP-1, IL-6 and procollagen type 1, were analyzed using PCR with human keratinocyte cells (HaCaT). Materials and methods Material Silver nitrate (AgNO3), crystal violet, 3-(4,5-dimethylthi- azol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulphoxide (DMSO) were purchased from Sigma- Aldrich chemicals (St. Louis, MO). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Gibco BRL (Grand Island, NY, USA). ELISA kits for MMP-1 and IL-6 were purchased from R&D Systems (R&D Systems Inc., Minneapolis, MN, USA). All other chemicals were of rea- gent grade. Organic solvents were purchased from Sam- chun chemicals (Korea). Inorganic salts were purchased from Sigma-Aldrich (St. Louis, MO, USA). Unless other- wise mentioned, solvents were purchased from Samchun chemicals (Seoul, Korea). In the present study, a total of seven plant extracts (Table S1) have been used to synthesize AgNPs. The selection of these plants was depending on the medicinal importance and previous study that has been per- formed. Dried plants were purchased from mountain rose herbs (Eugene, Oregon, USA). Preparation of aqueous leaf extract 10 g of the dried plants (root, leaf, seeds) was mixed with 100 mL of deionized water and autoclaved for 30 min at 100 °C. The aqueous extract was subsequently centrifuged at 10,000 rpm for 10 min to remove the debris. Finally, the supernatant obtained was filtered through a 0.45 μm PVDF syringe filter (SmartPor, Seoul, Korea). This plant extracts were used for further AgNP synthesis. In the present study, all the plant used for the synthesis of AgNPs are listed in Table S1. Synthesis of silver nanoparticles The AgNPs were synthesized as reported previously [20]. Briefly, aqueous plant extracts were diluted in water to 1:5 (v/v), to this solution the final concentration of 1 mM filter- sterilized solution of AgNO3 (Sigma-Aldrich chemicals, St. Louis, MO) has been added. The reaction mixture was kept at 65 °C. The synthesis was monitored for a change in the color, which indicated the synthesis of AgNPs. The AgNPs were collected by high-speed centrifugation at 20,000 rpm for 10 min. The obtained pellet was washed three times with distilled water to remove the unconverted metal ions or any other constituents. Characterization of silver nanoparticles UV–Vis spectrophotometer (UV–Vis) (Optizen POP; Mecasys; Daejeon, Korea) was used to confirm the reduction of metal ions and was scanned in the range of 300–800 nm. The transmission electron microscopy (TEM), elemental mapping, energy-dispersive X-ray spectroscopy (EDX) and selected area electron diffraction (SAED) analysis was per- formed through field emission transmission electron micros- copy (FE-TEM), with a JEM-2100F (JEOL, Tokyo, Japan) instrument operated at 200 kV. The sample for FE-TEM was prepared by placing a drop of collected nanoparticles on the carbon-coated copper grid and subsequently drying it at room temperature before transferring it to the microscope. The hydrodynamic size and zeta potential for AgNPs were measured in triplicate using Zetasizer Nano ZS90 (Malvern Instruments, UK), double-distilled water (DDW) was used as a dispersive medium. For DLS and zeta potential analysis, the samples were suspended in water and then used. The X-ray diffraction (XRD) analysis was performed on X-ray diffractometer, D8 Advance, (Bruker), Germany, operated at 40 kV, 40 mA, with CuKα radiation, at a scanning rate of 6° min−1, step size 0.02, over the 2θ range of 20°–80°. The functional groups capped on the surface of AgNPs were identified using a Fourier transform infrared (FT-IR) spec- troscopy (Spectrum One System, Perkin-Elmer, Waltham, MA). For XRD and FT-IR, the purified nanoparticles were dried and obtained in powder form then used. Antioxidant activity Free-radical-scavenging activity was measured by diphenyl- 1-picrylhydrazyl (DPPH) assay as described previously [21]. A 20 μL sample in distilled water was placed in a 96-well plate and 180 μL of 2, 2-diphenyl-1-picrylhydrazyl (0.2 mM) in methanol was also added. After a 30-min reaction in dark 13PDF Image | green silver nanoparticles from leaf used as UVb protection
PDF Search Title:
green silver nanoparticles from leaf used as UVb protectionOriginal File Name Searched:
Role_of_green_silver_nanoparticles_synthesized_fro.pdfDIY PDF Search: Google It | Yahoo | Bing
Turbine and System Plans CAD CAM: Special for this month, any plans are $10,000 for complete Cad/Cam blueprints. License is for one build. Try before you buy a production license. More Info
Waste Heat Power Technology: Organic Rankine Cycle uses waste heat to make electricity, shaft horsepower and cooling. More Info
All Turbine and System Products: Infinity Turbine ORD systems, turbine generator sets, build plans and more to use your waste heat from 30C to 100C. More Info
CO2 Phase Change Demonstrator: CO2 goes supercritical at 30 C. This is a experimental platform which you can use to demonstrate phase change with low heat. Includes integration area for small CO2 turbine, static generator, and more. This can also be used for a GTL Gas to Liquids experimental platform. More Info
Introducing the Infinity Turbine Products Infinity Turbine develops and builds systems for making power from waste heat. It also is working on innovative strategies for storing, making, and deploying energy. More Info
Need Strategy? Use our Consulting and analyst services Infinity Turbine LLC is pleased to announce its consulting and analyst services. We have worked in the renewable energy industry as a researcher, developing sales and markets, along with may inventions and innovations. More Info
Made in USA with Global Energy Millennial Web Engine These pages were made with the Global Energy Web PDF Engine using Filemaker (Claris) software.
Infinity Turbine Developing Spinning Disc Reactor SDR or Spinning Disc Reactors reduce processing time for liquid production of Silver Nanoparticles.
| CONTACT TEL: 608-238-6001 Email: greg@infinityturbine.com | RSS | AMP |