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Journal of Nanostructure in Chemistry https://doi.org/10.1007/s40097-018-0281-6 ORIGINAL RESEARCH Role of green silver nanoparticles synthesized from Symphytum officinale leaf extract in protection against UVB‐induced photoaging Hina Singh1,2 · Juan Du1,3 · Priyanka Singh1,4 · Tae Hoo Yi1 Received: 8 July 2018 / Accepted: 29 August 2018 © The Author(s) 2018 Abstract The present study demonstrated the simple, cheap, eco-friendly synthesis of the silver nanoparticles (S-AgNPs) using Sym- phytum officinale leaf extract. The biosynthesized S-AgNPs were characterized by UV–Vis, FE-TEM, elemental mapping, EDX, zeta potential, XRD, SAED, and FT-IR. The characterization results revealed the irregular shape and relatively stable nature of synthesized S-AgNPs. The average particle size was determined to be 87.46 nm. The zeta potential shows the negative surface charge (− 25.5 mV) of S-AgNPs. After characterization, we investigated the anti-aging effect of S-AgNPs in HaCaT keratinocyte cells. HaCaT keratinocyte cells were treated with S-AgNPs at concentrations 1, 10, 100 μg mL−1 after UVB or non-UVB irradiation. The S-AgNPs significantly inhibited the production of matrix metalloproteinase-1 and IL-6 but increased the expression of procollagen type 1. The data suggest that S-AgNPs have photoprotective properties and may have potential to be used as an agent against photoaging. Graphical abstract Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40097-018-0281-6) contains supplementary material, which is available to authorized users. Extended author information available on the last page of the article Vol.:(01233456789)PDF Image | green silver nanoparticles from leaf used as UVb protection
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