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Plants 2022, 11, 2379 Plants 2022, 11, x FOR PEER REVIEW 6 of 18 are means of four replicates ± SE. Different small letters (a–e) on bars denote significant difference of four replicates ± SE. Different small letters (a–e) on bars denote significant difference at p ≤ 0.05 at p ≤ 0.05 (Duncan’s multiple range test). Values sharing same letter within each subfigure are (Duncan’s multiple range test). Values sharing same letter within each subfigure are non-significant non-significant statistically. statistically. 2.6. Non-Enzymatic Antioxidants Theacttiiviittiiessooffnonno-ne-neznyzmymataictiacnatinotxioidxaidnatsn,tis.e,.i,.lee.,afleaanfdarnodotrgoloutagthluiotanteh(iotonteal,(tGoStaHl,, GSHSG,)G(FSiSgGur)e(3F)i,gAusrAe,3α)-,toAcospAh,erαo-ltoancodpthoetraollphaenndolitcosta(Fligpuhren4o)liwcsere(Fsiigunriefic4a)ntlwyerne- shiagnciefidcauntdlyeresnahliannitcyedstruenssd.eUrnsdaelirnsiatylinsitryesstr.eUssn,dmearxismaluinmityleasftraensds,rmooatxtiomtaulmglulteatfhiaonde r(2o1o%t t,o1t4a%l g)lcuotantheinotnsew(e2r1e%o,b1s4e%rv)ecdonintegnetsnowtyerpeeoBb-s1e0r0v0e8d, winhgilenrootoytpteoBta-l1g00lu0t8a,twhihoinle rcoont- toentatlsgolfusteanthsiotinve cgoentoetnytpseowf saesndsietcivreagsedno(1ty1p%e).wMasaxdiemcruemasleedaf(1a1n%d)r.oMotaGxiSmHum(23l%ea,f1a4n%d) raonodt rGoSoHt G(2S3S%G,(147%) aconndteronotst wGeSrSeGo(b1s7e%rv)ecdonintegnetnsowtyepre oBb-1s0er0v0e8d, winhiglenGoStyHpeanBd-1G0S00S8G, wcohnitlenGtSinHlaenadfaGnSdSrGoocotnotfeBn-t1i4n0l1e1afwaenrderdoeoctroeafsBe-d14t0o110w%eraende1c2re%a,seredstpoe1ct0i%vealyn,du1n2d%er, rseaslipneictytivsetrlyes,su(nFdigerusrael3in).itMy satxriemssu(mFigleuarfeα3-)t.oMcoapxihmeruoml (l1e0a9f%α)-tcoocnotpehnetrsowl (e1r0e9o%b)sceorvnetednitns wB-e1r0e0o08b,sewrvhedreians,Bi-n10th00e8r,owothtehreahsi,gihnetshteαr-otoctotphheehrioglh(e6s4t%α)-tcocnotpehnetsrowle(6re4%d)etceocntetednitns B-14011 under salinity stress. Similarly, the genotypes B-14011 accumulated highest total were detected in B-14011 under salinity stress. Similarly, the genotypes B-14011 6 of 18 150 120 90 60 30 0 Control 200 mM NaCl 200 ppm Si 200 mM NaCl + 200 ppm Si Leaf 100 80 60 40 20 0 Control 200 mM NaCl 200 ppm Si 200 mM NaCl + 200 ppm Si Root A d e f bc a b d cd B a b cc cd de e b 2 1.6 1.2 0.8 0.4 0 C de d a bb c B-10008 B-14011 Barley genotypes e de 1 0.8 0.6 0.4 0.2 0 D a b cd cd B-10008 B-14011 Barley genotypes b c dd −1 Figure2.Ascorbateperoxidase(APX)(A,B)(Umg−1 pprrooteteinin))aannddgglluttaatthiiooneerreduccttasse((GR))((C,,D) −1 −1−1 (μmol gg NADPHexext.t.mminin ))cocnotnetnetnstsinilnealfeanfdarnodotrofobtarolfeybgaerlneoytygpensomtyopdeuslamteoddbuylaatpedplibcay- application of silicon through rooting media under salinity stress. The values represented by bars tion of silicon through rooting media under salinity stress. The values represented by bars are means phenolics [leaf (30%) and root (38%)] while B-10008 accumulated maximum AsA [leaf (78%) accumulated highest total phenolics [leaf (30%) and root (38%)] while B-10008 and root (27%)] contents in response to salty stress as compared to control plants (Figure 4). accumulated maximum AsA [leaf (78%) and root (27%)] contents in response to salty Just like enzymatic antioxidants, the activities of non-enzymatic antioxidants were stress as compared to control plants (Figure 4). also enhanced after application of Si through rooting media in barley plants subjected to Just like enzymatic antioxidants, the activities of non-enzymatic antioxidants were salinity stress, except for root GSSG contents, which were lowered under salinity stress in also enhanced after application of Si through rooting media in barley plants subjected to B-14011. The results revealed that as compared to saline treatment the genotypes B-10008 salinity stress, except for root GSSG contents, which were lowered under salinity stress in accumulated maximum leaf total glutathione (13%), GSH (15%) and GSSG (6%) after B-14011. The results revealed that as compared to saline treatment the genotypes B-10008 application of Si through rooting media. Similarly, maximum root total glutathione (14%) accumulated maximum leaf total glutathione (13%), GSH (15%) and GSSG (6%) after application of Si through rooting media. Similarly, maximum root total glutathione (14%) GR (μmol g-1 NADPH ext. min.-1) APX (U mg-1 protein) GR (μmol g-1 NADPH ext. min.-1) APX (U mg-1 protein)PDF Image | Silicon-Induced Mitigation of NaCl Stress in Barley
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