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Chapter 6 – Production of Few Layer Graphene in Aqueous Media for Biological Studies Figure 6.18: a) Change in IPSD size profile upon addition of 0.1 v/v% G/DMF. The fluorescence quenching of Tyr and Trp (b) and Trp(c) alone by addition of different concentration of 0.1 v/v% G/DMF excited at 280 nm and 295 nm, respectievly. d) Stern-Volmer plot of 0.1 v/v% G/DMF quenching Tyr and Trp residues of HSA. The Stern-Volmer plot (Figure 6.18d) shows an upward curvature which indicates the quenching mechanism is combined static and dynamic quenching or predominantly static quenching.415, 429-431 It has been determined that, for GO, the main quenching mechanism is static quenching with some contribution of dynamic quenching, which is most likely the reason here as both electrostatic and hydrophobic interactions play a role in stabilising the graphene.415 191PDF Image | PRODUCTION AND APPLICATIONS OF GRAPHENE AND ITS COMPOSITES
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